Practical HPLC Method Development

For the experienced chromatographer, this course provides a step-by-step approach to method development.

The course includes all the crucial aspects of method development, including information gathering, mode of chromatography, development of mobile phase systems, column choice, principles of ionisation/suppression, optimisation of important chromatographic parameters (R, α, k’ and N), isocratic and gradient operation and detector choice and optimisation

Each aspect is discussed in detail with relevant examples used to demonstrate theoretical principles. Chromatographic modelling software-based exercises are included to give further understanding, and practical laboratory sessions are included to make an all encompassing two day course.

  • We limit numbers to 6 per course so that each delegate gets the opportunity to ask questions and fully participate in practical exercises
  • When delivered on-site we can design the course material to suit your specific training needs
  • Customisable written assessments are available if required

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